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Fedbatch - Prozessoptimierung
P. pastoris mit Substratmischungen
Data
V:\Data-Monat\Data-Februar\hyp\Experiments_Data
Maurer, M., M., Kühleitner, B. Gasser, and D. Mattanovich. 2006. Versatile modeling and optimization of fed  batch processes for the production of secreted heterologous proteins with Pichia pastoris. Microbial Cell Factories. 5: 37.
102 kBxls
 
References
Hans-Peter Meyer, 7. Juni 2007
1307 kBpdf
Cereghino, J.L., and J.M. Cregg. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24: 45-66.
483 kBpdf
Curvers, S., P. Brixius, T. Klauser, J. Thoemmes, B.D. Weuster, R. Takors, and C. Wandrey. 2001. Human chymotrypsinogen B production with Pichia pastoris by integrated development of fermentation and downstream processing. Part 1. Fermentation. Biotechnol. Prog. 17: 495-502.
213 kBpdf
d’Anjou, M.C., and A.J. Daugulis. 2001. A rational approach to improving productivity in recombinant Pichia pastoris fermentation. Biotechnol. Bioeng. 72: 1–11.
214 kBpdf
Invitrogen. 2002. Pichia Fermentation Process Guidelines. Catalog No. K1710-01, Version B 053002, 1-11. http://www.invitrogen.com/content/sfs/manuals/pichiaferm_prot.pdf
162 kBpdf
Maurer, M., M., Kühleitner, B. Gasser, and D. Mattanovich. 2006. Versatile modeling adn optimization of fed  batch processes for the production of secreted heterologous proteins with Pichia pastoris. Microbial Cell Factories. 5: 37.
323 kBpdf
Minning, S., A. Serrano, P. Ferrer, C. Solá, R.D. Schmid, and F. Valero. 2001. Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. J. Biotechnol. 86: 59–70.
196 kBpdf
Zhang, W., J. Sinha, L. A. Smith, M. Inan, and M.M. Meagher. 2005. Maximization of production of secreted recombinant proteins in Pichia pastoris fed batch fermentation. Biotechnol. Prog. 21: 386-393.
195 kBpdf
 
Models
 
 

 
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Nomenclature

Abbreviation
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  A  B  C  D  E  F  G  H  I  J  K  L  M  N  O  P  Q  R  S  T  U  V  W  X  Y  Z  
Δt [min] Zeitintervall
CPR [mol h-1] Kohlenstoffdioxidproduktionsrate
carbon dioxide production rate (CO2 production rate)
cCO2 [%] Kohlenstoffdioxidkonzentration in der Prozessabluft
concentration of carbon dioxide in the exhaust air
cCO2Air [%] Kohlenstoffdioxidkonzentration in der Prozesszuluft
concentration of carbon dioxide in the supply air
ci [g L-1] Konzentration des Stoffes i im Behälter (und im Abfluss)
concentration of component i in the reactor (and its outflow)
cin,i,h [g L-1] Konzentration des Stoffes i im Zufluss h
concentration of component i in the inflow stream
cO2 [%] Sauerstoffkonzentration in der Prozessabluft
concentration of oxygen in the exhaust air
cO2Air [%] Sauerstoffkonzentration in der Prozesszuluft
concentration of oxygen in the supply air
cp [g L-1] Produktkonzentration
concentration of product
D [h-1] Verdünnungsrate
dilution rate
Dcrit [h-1] kritische Verdünnungsrate (Auswaschpunkt)
critical dilution rate (washout)
Dopt [h-1] optimale Verdünnungsrate (bei maximaler Produktivität)
dilution rate for optimum/maximum productivity
DT [min] D-Wert
Fair [L min-1] Luftstrom
volumetric flow of air
Fin [g h-1] Dosierrate
feed rate
Fin,h [L h-1] Volumenstrom Zufluss h
volumetric flow rate of the inflow stream
Fout,k [L h-1] Volumenstrom Abfluss k
volumetric flow rate of the outflow stream
FT [min] F-Wert
h [-] Anzahl Zuflüsse, h=1...nin
number of inflows, h=1...nin
i [-] Anzahl Stoffe, i=1...n
number of components, i=1...n
j [-] Anzahl Reaktionsprozesse, j=1...m
number of processes, j=1...m
Ki [g L-1] Inhibitionskonstante für Wachstum
inhibition constant of growth
Km [g L-1] Michaelis-Menten Konstante
Michaelis-Menten constant
Ks [g L-1] Substrataffinitäts-/Sättigungskonstante (Monod)
substrate affinity/saturation constant (Monod)
KT [-] thermische Abtötungskonstante (Basis "10"), wobei K = kT/2303
k [-] Anzahl Abflüsse, k=1...nout
number of outflows, k=1...nout
kd [h-1] spezifische Absterberate
specific death rate
kn [g1-n L1-n h-1] Konstante für Kinetik n-ter Ordnung
rate constant of n-order kinetics
kT [-] thermische Abtötungskonstante (Basis "e")
L [min] Letalrate, Letalitätsfaktor
mi [g] Menge des Stoffes i im Behälter
amount of component i in the reactor
ms [g g-1 h-1] spezifische Rate des Substratverbrauchs für Zellerhaltung
specific maintainance rate for substrate
µ [h-1] spezifische Wachstumsrate
specific growth rate
µmax [h-1] maximale spezifische Wachstumsrate
maximum specific growth rate
N [ml-1] Anzahl Organismen/Zellen
number of organisms/cells
N' [ml-1] Anzahl der abgetöteten Keime
N0 [ml-1] Anfangsanzahl der Organismen/Zellen (Inokulum)
initial number of organisms/cells (inoculum)
n [-] Anzahl Generationen
number of generations
OUR [mol h-1] Sauerstoffverbrauchsrate
oxygen uptake rate
p [g L-1] Produktkonzentration im Behälter
product concentration in the reactor
p0 [g L-1] Anfangsproduktkonzentration
initial product concentration
pO2 [%] relativer Sauerstoffpartialdruck
relative oxygen partial pressure
Qm/p [%w/v] o. [%n/n] Ausbeutekoeffizient Metabolit/Substrat
yield coefficient (metabolite/substrate)
qs [g g-1 h-1] spezifische Substratverbrauchsrate
specific rate of substrate consumption
qs, max [g g-1 h-1] maximale spezifische Substratverbrauchsrate
maximum specific substrate consumption rate
ρ [kg L-1] Dichte
density
Rj [g L-1 h-1] volumetrische Prozessgeschwindigkeit für Prozess j
volumetric conversion rate for process j
RQ [mol O2 (mol CO2)-1] respiratorischer Quotient
respiratory quotient
ri [g L-1 h-1] volumetrische Umwandlungsgeschwindigkeit
volumetric conversion rate
rpm [rpm] Rührerdrehzahl
rotations per minute
rs [g L-1 h-1] Substratverbrauchsrate
rate of substrate consumption
rx [g L-1 h-1] Biomassebildungsrate (Biomasseproduktivität)
rate of biomass production (volumetric biomass productivity)
s [g L-1] Substratkonzentration im Behälter
concentration of substrate in the reactor
s0 [g L-1] Anfangssubstratkonzentration
initial substrate concentration
sin [g L-1] Substratkonzentration im Medienzufluss
substrate concentration in the inflow stream
smin [g L-1] Substratkonzentration bei µ = 0 h-1 (Schwellenkonzentration)
concentration of substrate at µ = 0 h-1 (threshold)
s·V [g] Substrat (Masse)
(mass of) substrate
T [°C] o. [K] Temperatur
temperature
Ti [°C] Messtellentemperatur
t [h] Zeit
time
td [h] Generationszeit (Verdopplungszeit)
time of generation (doubling time)
V [L] aktuelles Volumen (auch als Arbeitsvolumen VR bez.)
volume (also working volume VR)
V0 [L] Anfangsreaktorvolumen
initial reactor volume
VM [L mol-1] molares Volumen (Molvolumen )
molar volume
w [%] Anteil Substrat in der zudosierten Substratösung
fraction of sbstrate in feed solution
x [g L-1] Biomassekonzentration im Behälter
concentration of biomass in the reactor
x0 [g L-1] Anfangsbiomassekonzentration (Inokulum)
initial biomass concentration (inoculum)
xmax [g L-1] maximale Biomassekonzentration
maximum biomass concentration
x·V [g] Biomasse (Masse)
(mass of) biomass
Yp/s [g g-1] o. [mol mol-1] Ausbeutekoeffizient Produkt/Substrat
yield coefficient (product/substrate)
Yp/x [g g-1] o. [mol mol-1] Ausbeutekoeffizient Produkt/Biomasse
yield coefficient (product/biomass)
Yx/C [g g-1] o. [mol mol-1] Ausbeutekoeffizient Biomasse/Kohlenstoff in Substrat
yield coefficient (biomass/carbon of substrate)
Yx/s [g g-1] o. [mol mol-1] Ausbeutekoeffizient Biomasse/Substrat
yield coefficient (biomass/substrate)
z [K] z-Wert
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