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Tools: Simulation Chemostat
Topic 1: Batch growth
  Task description
  Varma and Palsson (1994)
  Kovarova-Kovar and Egli (1998)
  Excel Data (Varma and Palson,1994)
 Topic 2:
Continuous culture - x(t), s(t) graphs vs. x(D), s(D) diagrams
  Task description
  Kayser et al. (2005)
  Ihssen and Egli (2004)
  Excel Data (Kayser et al., 2005)
  Excel Data (Ihssen and Egli, 2004)
Topic 3: Transition from batch to continuous culture
  Task description
Topic 4: Batch growth - interpretation of experimental data
  Task description
  Tao et al. (1998)
Topic 5:  Continuous culture - interpretation of experimental data
   Task description
   Kovarova et al. (1996)
Topic 6: Transients - Interpretation of the experimental data
  Task description
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230 kBpdf
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Egli, T., J.P. van Dijken, M. Veenhuis, W. Harder, and A. Fiechter. 1980. Methanol Metabolism in Yeasts: Regulation of the Synthesis of Catabolic Enzymes. Arch. Microbiol. 124: 115-121.
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Hans-Peter Meyer, 7. Juni 2007
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Ihssen, J., and T. Egli. 2004. Specific growth rate and not cell density controls the general stress response in Escherichia coli. Microbiol. 150: 1637 – 1648.
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Invitrogen. 2002. Pichia Fermentation Process Guidelines. Catalog No. K1710-01, Version B 053002, 1-11. http://www.invitrogen.com/content/sfs/manuals/pichiaferm_prot.pdf
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Lendenmann, U., H. Senn, M. Snozzi, and T. Egli. 2000. Dynamics of Mixed Substrate Growth of Escherichia coli in Batch Culture: the Transition between Simultaneous and Sequential Utilisation of Carbon Substrates. Acta Univ. Carol. Environ. 14: 21-31.
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Minning, S., A. Serrano, P. Ferrer, C. Solá, R.D. Schmid, and F. Valero. 2001. Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. J. Biotechnol. 86: 59–70.
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Papoutsakis, E.T. 1984. Equations and Calculations for Fermentations of Butyric Acid Bacteria. Biotechnol. Bioeng. XXVI: 174-187.
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Piñar, G., K. Kovářová, T. Egli, and J.L. Ramos. 1998. Influence of Carbon Source on Nitrate Removal by Nitrate tolerant Klebsiella oxytoca CECT 4460 in Batch and Chemostat Cultures. Appl. Env. Microbiol. 64: 2970 - 2976. 
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van Dijken, J.P., Otto, R., and W. Harder. 1976. Growth of Hansenula polymorpha in a methanol-limited chemostat. Arch. Microbiol. 111: 137-144.
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Varma, A., and B.O. Palsson. 1994. Stoichiometric Flux Balance Models Quantitatively Predict Growth and Metabolic By-Product Secretion in Wild-Type Escherichia coli W3110. Appl. Environ. Microbiol. 60: 3724-3731.
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von Meyenburg, K. 1971. Transport-Limited Growth Rates in a Mutant of Escherichia coli. J. Bacteriol. 107: 878-888.
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Wanner, U., and T. Egli. 1990. Dynamics of microbial growth and cell composition in batch culture. FEMS Microbiol. Rev. 75: 19-44.
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Zhang, W., J. Sinha, L. A. Smith, M. Inan, and M.M. Meagher. 2005. Maximization of production of secreted recombinant proteins in Pichia pastoris fed batch fermentation. Biotechnol. Prog. 21: 386-393.
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